Bio::Tools::Primer3Redux::PrimerPair
# get the primer pair from the Bio::Tools::Primer3Redux::Result my $pair = $result->next_primer_pair; # grab left/right primers my ($fp, $rp) = ($pair->forward_primer, $pair->reverse_primer);
Bio::Tools::Primer3Redux::PrimerPair acts as a simple SeqFeature that bundles primer pair data together into one object. This object can be used to retrieve the amplicon sequence, the forward/reversion (left/right) primers, and any internal oligos. Furthermore, any primer information relative to the product is included as SeqFeature tags.
Bio::Tools::Primer3Redux::PrimerPair - Simple Decorator of a Bio::SeqFeature::Generic with convenience methods for retrieving left and right primers, internal oligos, and any amplicon-related information
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The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
Title : left_primer Note : Alias of forward_primer()
Title : forward_primer Usage : $obj->forward_primer Function : returns the forward (left) primer Returns : Bio::Tools::Primer3Redux::Primer Args : Optional Bio::Tools::Primer3Redux::Primer
Title : right_primer Note : alias of reverse_primer()
Title : reverse_primer Usage : $obj->reverse_primer Function : returns the reverse (right) primer Returns : Bio::Tools::Primer3Redux::Primer Args : Optional Bio::Tools::Primer3Redux::Primer
Title : internal_oligo Usage : $obj->internal_oligo Function : returns the internal oligo (if present) Returns : Bio::Tools::Primer3Redux::Primer Args : Optional Bio::Tools::Primer3Redux::Primer
cjfields <cjfields@bioperl.org>
This software is copyright (c) 2011 by Chris Fields.
This is free software; you can redistribute it and/or modify it under the same terms as the Perl 5 programming language system itself.
To install Bio::Tools::Primer3Redux, copy and paste the appropriate command in to your terminal.
cpanm
cpanm Bio::Tools::Primer3Redux
CPAN shell
perl -MCPAN -e shell install Bio::Tools::Primer3Redux
For more information on module installation, please visit the detailed CPAN module installation guide.